A three-domain copper-nitrite reductase with a unique sensing loop

A Copper Nitrosyl in Nitrite Reductase

Directed evolution of copper nitrite reductase to a chromogenic reductant.

Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput screen includes colony lift onto a nylon membrane and subsequent lysis of NiR-expressing colonie...

A eukaryotic copper-containing nitrite reductase derived from a NirK homolog gene of Aspergillus oryzae.

We cloned a bacterial copper-containing nitrite reductase (NirK) homolog gene of Aspergillus oryzae (AonirK). Alignment showed that amino acid residues crucial for copper binding are conserved in the deduced sequence of the fungal protein. The recombinant protein exhibited distinct nitrite reductase activity, and its absorption and EPR spectra showed the presence of type 1 and type 2 copper ato...

Characterization of a novel copper-haem c dissimilatory nitrite reductase from Ralstonia pickettii.

NiRs (nitrite reductases) convert nitrite into NO in the denitrification process. RpNiR (Ralstonia pickettii NiR), a new type of dissimilatory Cu-containing NiR with a C-terminal haem c domain from R. pickettii, has been cloned, overexpressed in Escherichia coli and purified to homogeneity. The enzyme has a subunit molecular mass of 50515 Da, consistent with sequence data showing homology to th...

Modified Glass Carbon Electrode (GCE) Electropolymerized Polypyrrole Nanofibers with Hemoglobin (Hb) Film as a Unique Biosensor for Nitrite Determination

Abstract: In this study, we were investigated behavior the electrochemical reductionof nitrite at a hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containingpolypyrol nanofiber (ppy) films. Polypyrrole (PPy) nanofibers have been constructed onGCE applying electrochemical technique, and can to deposit diverse polymers onminiaturized electrodes with this commo...